133x Filetype PPT File size 1.68 MB Source: www.bio.utexas.edu
Fluorescence Microscopy “fluorescence imaging has become the mainstay of microscopy” -- Nat. Methods, 2005 Objectives: 1. Understand the basics of fluorescence 2. Understand how fluorophores are used in microscopy 3. Understand how a microscope is set up to do fluorescence 4. Understand basics of immunofluorescence Have you used a fluorescence microscope before? A. Yes B. No Fluorescence microscopy uses substances called “fluorophores” or “fluorochromes” as stains. These substances have the property that they “fluoresce”, i.e. they absorb some wavelength of light, become excited, and decay to the ground state by emitting a slightly longer wavelength of light. R O Y G B I V 700 650 600 550 500 450 400 wavelengths in nm E = h
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