jagomart
digital resources
picture1_Light Microscopy Slideshare 77715 | Wameedmuclecture 2021 92831981


 232x       Filetype PPTX       File size 0.39 MB       Source: www.uomus.edu.iq


File: Light Microscopy Slideshare 77715 | Wameedmuclecture 2021 92831981
a fluorescence microscope is an optical microscope that uses fluorescence and phosphorescence instead of or in addition to reflection and absorption to study organic or inorganic substances fluorescence is the ...

icon picture PPTX Filetype Power Point PPTX | Posted on 03 Sep 2022 | 3 years ago
Partial capture of text on file.
   A fluorescence microscope is an optical microscope 
  that uses fluorescence and phosphorescence instead of, 
     or in addition to, reflection and absorption to study  
  .                                  organic or inorganic substances
                                        
  Fluorescence is the emission of light by a substance 
  that has absorbed light or other electromagnetic 
  .radiation
  The fluorescence microscope was devised in the early 
   part of the twentieth century by August Köhler, Carl 
   Reichert, and Heinrich Lehmann.     
  Principle of Fluorescence Microscopy 
  Most cellular components are colorless and 
  cannot be clearly distinguished under a 
  microscope. The basic premise of fluorescence 
  .microscopy is to stain the components with dyes
  Fluorescent dyes, also known as fluorophores or  
  fluorochromes, are molecules that absorb 
  excitation light at a given wavelength (generally 
  UV), and after a short delay emit light at a longer 
  . wavelength
    The emission light can then be filtered from the 
      excitation light to reveal the location of the 
      fluorophores.
    Fluorescence microscopy uses a much higher 
      intensity light to illuminate the sample. This 
      light excites fluorescence species in the sample, 
      which then emit light of a longer wavelength.
    The image produced is based on the second light 
    source or the emission wavelength of the 
    fluorescent species rather than from the light 
    originally used to illuminate, and excite, the 
    sample
    Parts of Fluorescence Microscope         
    Fluorescent dyes (Fluorophore)- 1    
       A fluorophore is a fluorescent chemical compound that 
        can re-emit light upon light excitation.
       Fluorophores typically contain several com
       bined aromatic groups, or plane or cyclic molecules 
        with several π bonds.
       Many fluorescent stains have been designed for a range 
        of biological molecules.
       Some of these are small molecules that are intrinsically 
        fluorescent and bind a biological molecule of interest. 
        Major examples of these are nucleic acid stains like 
        DAPI and Hoechst, phalloidin which is used to stain 
        actin fibers in mammalian cells. 
The words contained in this file might help you see if this file matches what you are looking for:

...A fluorescence microscope is an optical that uses and phosphorescence instead of or in addition to reflection absorption study organic inorganic substances the emission light by substance has absorbed other electromagnetic radiation was devised early part twentieth century august kohler carl reichert heinrich lehmann principle microscopy most cellular components are colorless cannot be clearly distinguished under basic premise stain with dyes fluorescent also known as fluorophores fluorochromes molecules absorb excitation at given wavelength generally uv after short delay emit longer can then filtered from reveal location much higher intensity illuminate sample this excites species which image produced based on second source rather than originally used excite parts fluorophore chemical compound re upon typically contain several com bined aromatic groups plane cyclic bonds many stains have been designed for range biological some these small intrinsically bind molecule interest major exa...

no reviews yet
Please Login to review.