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picture1_Information Ppt 66811 | Scanning Spectrophotometry 1


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File: Information Ppt 66811 | Scanning Spectrophotometry 1
objectives 1 what is absorption spectrum and determination of max standard curve and determination of an unknown concentration for a 2 solution how a spectrophotometer works spectrophotometer components spectrophotometer it ...

icon picture PPTX Filetype Power Point PPTX | Posted on 28 Aug 2022 | 3 years ago
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   :Objectives
 1. What is absorption spectrum and determination of λmax.
Standard curve and determination of an unknown concentration  for a . 2 
.solution
           .How a spectrophotometer works
           (spectrophotometer components)
 Spectrophotometer: it can be used to measure the amount of light absorbed by a 
 solution. How?
 -By using the spectrophotometer, we can quantitatively measure absorbance, and 
 this information can be used to determine the concentration of the absorbing 
 molecule.
 -More concentrated solution will absorb more light and transmits less.[why?]
 So, the more concentrated solution  high absorbance value.
 And Less concentrated solution  less absorbance value. 
 Absorption spectrum:
 The curve that display the action or behaviour of absorption of  molecule [solute] at 
 different  weave lengths, Known as Absorption spectrum curve.
 -Every molecule has its own Absorption spectrum, So it’s  considered as fingerprint for 
 each molecule.
 λmax: it is the weave length at which the molecule has the  maximum absorbance at this 
 weave length.[best absorbance]. It can be determined from absorption spectrum curve. 
          [the wavelength of maximum absorption (λmax)]
  
         Absorption spectrum curve and determination of λmax.
                     
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...Objectives what is absorption spectrum and determination of max standard curve an unknown concentration for a solution how spectrophotometer works components it can be used to measure the amount light absorbed by using we quantitatively absorbance this information determine absorbing molecule more concentrated will absorb transmits less so high value that display action or behaviour at different weave lengths known as every has its own s considered fingerprint each length which maximum determined from...

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