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picture1_Animal Tissue Culture Pdf 88771 | 52d088bab1ac300e945a73dbdaabcd7d


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File: Animal Tissue Culture Pdf 88771 | 52d088bab1ac300e945a73dbdaabcd7d
microbiology culturemedia iv semestermsczoology midhila mohan assistant professor on contract animal cell and tissue culture culture media natural media and artificial media culture media the nutrient media used for culture ...

icon picture PDF Filetype PDF | Posted on 15 Sep 2022 | 3 years ago
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  Microbiology….
          culturemedia
          iv SemestermsCzoology
          MIDHILA MOHAN ASSISTANT PROFESSOR ON CONTRACT
        ANIMAL CELL AND TISSUE 
                CULTURE
          CULTURE MEDIA :Natural media and Artificial media
   CULTURE MEDIA
       The nutrient media used for culture of animal cells and  tissues
       It must be able to support their survival as well as growth i.e. must be provide nutritional and stromal factor
        culture media are divided  in to two . They are 
   NATURLAL MEDIA 
   ARTIFICIAL MEDIA
       The choice of medium depends mainly on the type of cells to be  cultured (normal ,immortalized or transformed)
       and the objective of culture (growth , survival, differentiation, production of desired protein 
         Non transformed or normal cells and primary cultures from 
         healthy tissues require defined quantities of proteins , growth 
         factors and hormones 
         Immortalized cells produce  most of these factors but may still 
         need some of the growth factors present In the serum 
          Transformed cells synthesize their on growth factors ., in fact 
         addition of growth factors may even the detrimental in such 
         cases . But even these cultures may require factors like insulin , 
         transferrin lipids etc.   
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...Microbiology culturemedia iv semestermsczoology midhila mohan assistant professor on contract animal cell and tissue culture media natural artificial the nutrient used for of cells tissues it must be able to support their survival as well growth i e provide nutritional stromal factor are divided in two they naturlal choice medium depends mainly type cultured normal immortalized or transformed objective differentiation production desired protein non primary cultures from healthy require defined quantities proteins factors hormones produce most these but may still need some present serum synthesize fact addition even detrimental such cases like insulin transferrin lipids etc...

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