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purdue extension fs 15 w food entrepreneurship series food preservation methods department of foods are freshest and at optimum quality at the food science time of their harvest or slaughter ...

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                                                                                                        Purdue extension
                                                                                                                                                     FS-15-W
                                                                    Food Entrepreneurship Series
                                          Food Preservation Methods                                                      Department of 
                                          Foods are freshest and at optimum quality at the                                      Food Science
                                          time of their harvest or slaughter. To maintain this       ous ways to extend the period of time during which 
                                          quality in foods that will be consumed later, the          the food can be shipped, displayed in the store, 
                                          foods can be preserved by cold, heat, chemical             purchased by the consumer, and finally consumed. 
       Katherine Clayton                  preservatives, or combinations of these methods.           The physical and chemical composition of the 
       food science Extension             Cold usually means refrigeration or freezing.              food helps determine the type of process required 
       outreach specialist                Heating involves many processing methods,                  for preservation. Other factors that influence a 
       Deidre Bush                        such as pasteurization, commercial sterilization,          choice of preservation method are the desired end 
       former Extension assistant         and drying. Adding preservative ingredients and            product, type of packaging, cost, and distribution 
                                          processing by means of fermentation are also               methods.
       Kevin Keener                       ways to preserve foods.                                    The Role of Water Activity and 
       food process engineer,             A food entrepreneur needs a basic understand-              Acidity in Preservation
       Extension specialist, and          ing of the various preservation techniques before 
       professor of food science          starting a business.                                       The two most important chemical composition 
                                          Food processing converts harvested or raw                  factors that affect how a food is preserved are 
                                          foods into forms that are more easily stored and           water content and acidity. Water content includes 
       Department of Food Science         consumed, and sometimes into a form that may               moisture level, but an even more important 
                                                                                                     measurement is water activity. Water activity (a ) 
       745 Agriculture Mall Drive         be more desirable. For example, wheat is pro-              refers to the energy status of water in the food,  w
       West Lafayette, IN  47907          cessed into flour, which is used to make bread             which affects whether or not chemical reactions 
       www.foodsci.purdue.edu             and pasta. Strawberries can be processed into              occur and/or microorganisms will grow. The 
                                          freeze-dried berries for use in cereals or cooked          content of the food—such as sugar, salt, protein, or 
                                          to create strawberry preserves.                            starch—“binds” the water, making it less available. 
                                          Foods can be generally classified as cereals,              Foods with lower water activities are less prone 
                                          fruits, vegetables, dairy, and meats. Different            to spoilage by microorganisms and have fewer 
                                          food types are preserved and processed in vari-            undesirable chemical changes occur during 
                                                                                                     storage. 
                                          Water activity levels for growth of microorganisms
                                              aw       Microorganisms that grow at this water activity level
                                                                  
                                            0.95             Salmonella spp., Pseudomonas, Escherichia coli, Bacillus cereus, some yeasts
                                            0.90             Clostridium botulinum, Lactobacillus, Listeria monocytogenes, Clostridium perfringens 
                                            0.87             Yeasts, Staphylococcus aureus
                                            0.80             Molds, Saccharomyces spp.
                                            0.60             Some yeasts and molds
         FS-15-W                Food Entrepreneurship Series  •                                         Purdue extension
                                Food Preservation Methods 
                        
          2                         Water activity levels of                                      Federal and state food regulations specify that a 
                                    common foods                                                  shelf-stable product that does not require refrigera-
                                     aw range           Food                                      tion or does not undergo sufficient heat treatment 
                                    0.95–0.99              Fresh meat, fish                       must have a water activity equal to or below 0.85 or 
                                    0.90–0.95              Bread                                  a natural pH equal to or below 4.6. Various changes 
                                    0.85–0.95              Cheese                                 can be made to product recipes in order to reach the 
                                    0.80–0.91              Jams                                   target water activity and/or pH level. For example, 
                                    0.75–0.90              Honey, syrups                          adding sugar or salt to a product can lower its water 
                                    0.60–0.90              Cakes, pastry                          activity. And adding acid—in the form of vinegar 
                                    0.60–0.75              Dried fruits                           or lemon juice can reduce pH. Reducing the water 
                                    0.20–0.35              Crackers                               activity below 0.85 or acidifying a food to reach a 
                                                                                                  pH level of 4.6 or lower will prevent the growth of 
                                   The water activity of pure water is 1.0 (or 100%               harmful bacteria. Adding preservative compounds—
                                   relative humidity). A dry cracker has a water activity         such as sodium benzoate (to prevent bacteria and 
                                   of about 0.2, and jam has a water activity of about            yeast growth) or potassium sorbate (to prevent yeast 
                                   0.85. A low level for water activity indicates less free       and mold growth)—can also aid in the preservation 
                                   water in the food. Neither Staphylococcus aureus nor           process.
                                   any other pathogen can grow at a water activity level          Preservation Techniques
                                   of 0.85 or below.                                              Foods are often preserved with a combination of 
                                   Acidity refers to pH, or level of hydrogen ions,               formulation (added ingredients), processing (heating 
                                   which is measured on a scale of 0–14. Products                 or cooling), and distribution methods (shelf stable, 
                                   with a low pH (below 7.0) are acidic, and products             refrigeration, freezing). When choosing the best 
                                   with a high pH (above 7.0) are alkaline (basic). For           method for preserving a product, food processors 
                                   example, tomatoes have a pH range of 4.1–4.9, so               must pay close attention to the pH and water activity 
                                   they are acidic. Egg whites have a pH range of 7.6-            while also considering how a preservation technique 
                                   9.6, so they are alkaline. A pH of 7.0 is considered           will affect the quality of the finished product.  
                                   neutral; water has a pH of 7.0. A pH level below 4.6           Preservation techniques commonly used for food 
                                   inhibits the production of a deadly toxin produced by          processing are outlined below. More detailed de-
                                   Clostridium botulinum, which causes botulism.                  scriptions of these commercial processing methods 
                                                                                                  can be found in textbooks.
            pH levels for growth of microorganisms                                                Refrigerating
            pH range of growth                       Microorganism                                Potentially hazardous foods, or those foods with a 
            5.5–8.0                                           Clostridium perfringens             pH greater than 4.6 and water activity greater than 
            4.9–9.3                                           Bacillus cereus                     0.85, must be kept below 40˚F. These foods include 
            4.6–9.5                                           Escherichia coli                    cooked meat and poultry, milk and dairy products, 
                                                                                                  eggs, products made with eggs, shellfish, and sea-
            4.5–9.0                                           Salmonella spp.                     food. Foods that cannot be dried or canned or that 
            4.2–9.0                                           Clostridium botulinum               need to maintain a fresh quality are also preserved 
            4.2–9.3                                           Staphylococcus aureus               by refrigeration. Examples are perishable fruits and 
           pH levels of common foods                                                              vegetables, meat and poultry, cheese, yogurt, fresh 
                                                                                                  salsa, and soy milk. These products have a limited 
           pH range                                    Food                                       shelf life because refrigeration only slows bacterial 
           7.1–7.9                                              Eggs                              growth; it does not prevent it. Mishandling during 
           6.3–8.5                                              Milk                              shipment and by the consumer can increase the rate 
           5.3–5.8                                              Bread                             of bacterial growth and should be accounted for in 
           5.0–7.0                                              Meats                             shelf-life determination. Additionally, these prod-
           4.8–7.3                                              Fish                              ucts have a higher warehousing and shipping cost 
           4.0–7.0                                              Vegetables                        because of the need to maintain proper refrigera-
                                                                                                  tion. However, these products have high appeal to 
           3.3–7.1                                              Fruits                            consumers because they are fresh and convenient.
           3.1–4.5                                              Berries
           FS-15-W                   Food Entrepreneurship Series  •                                                   Purdue extension
                                     Food Preservation Methods 
                          
           3                           Freezing                                                                 flavor and color. This process is done to ensure 
                                       Freezing can be used to preserve a number of food                        the treated food is safe for human consumption. 
                                       products. Commercially frozen food is stored at -10˚F                    Pasteurization is most commonly used on liquids 
                                       to 20˚F. Freezing halts bacterial growth, but does not                   such as milk and juices, with raw milk being the 
                                       eliminate bacteria. If processed carefully, a frozen                     most common pasteurized food. High-temperature/
                                       food product will maintain quality in color, texture, and                short-time pasteurized milk is heated for 15 seconds 
                                       flavor for a long shelf life. Frozen foods, such as large                at 161˚F. Ultra-high-temperature pasteurized milk is 
                                                                                                                                                    . These different time/
                                       cuts of meat that require thawing, are less convenient                   heated for 2 seconds at 280˚F
                                       than fresh foods. However, consumers perceive food                       temperature treatments for milk are equally effective 
                                       items such as frozen meals and desserts as more                          at reducing harmful bacteria and many spoilage 
                                       convenient than making them from scratch at home.                        microbes. In addition to making a product safer for 
                                       In addition, frozen fruits and vegetables are perceived                  human consumption, pasteurization also increases 
                                       as being fresher than canned. As with refrigeration,                     product shelf life. Most pasteurized products are 
                                       commercially frozen foods have the disadvantage of                       stored under refrigeration conditions and are not 
                                       higher storage and distribution costs in addition to the                 shelf stable.
                                       energy costs to initially freeze the food product.                       Thermal Processing (Low-Acid  
                                       Drying (Traditional, Freeze-Dry, Spray-Dry)                              Canned Foods)
                                       Dehydrated foods have a long shelf life because                          Foods that are stored at room temperature and 
                                       removal of moisture lowers the water activity to below                   sold in a sealed container (metal, glass, or plastic 
                                       0.50 so that spoilage organisms cannot grow. Fruits                      laminate pouch) are heat processed to destroy 
                                       and vegetables can be dried and sold as is, or they                      microorganisms that can either spoil the food or 
                                       can be used in other dry products that have a long                       cause a health hazard. These thermally processed 
                                       shelf life, such as cereal or granola bars. Traditional                  foods are called  “commercially sterile”—or, more 
                                       drying of foods utilizes heat, air, and time in various                  commonly, “shelf stable.” The time and temperature 
                                       processes that allow removal of moisture to a desired                    needed to make a food shelf stable depends on 
                                       level. Freeze-drying is a form of dehydration in which                   several factors, including the pH and physical nature 
                                       the product is frozen and water is removed as vapor. 
                                       Spray-drying is a method that rapidly dries a liquid 
                                       slurry by spraying small droplets into a heated cham-
                                       ber. Milk that undergoes spray-drying is sold as pow-
                                       dered milk that can be reconstituted. The reduction of 
                                       moisture content by heat treatment in drying can be 
                                       expensive, depending on the time required. Addition-
                                       ally, there is usually some loss of product quantity 
                                       and quality associated with any drying method.
                                       Pasteurizing
                                       Pasteurization uses heat treatment for a short time 
                                       to destroy harmful microorganisms that might be 
                                       in a food without adversely affecting the food’s 
                                       Pasteurization Methods
                                           Product            Temperature                   Time
                                                                     145˚F                 30 min.
                                           Milk                      161˚F                 15 sec.
                                                                     280˚F                  2 sec.
                                           Juice                     155˚F                 30 min.              When doing any type of thermal processing, it 
                                                                     180˚F                 15 sec.              is important to verify the final temperature of 
                                           Egg in shell              130˚F                 45 min.              the product. Here a kitchen manager checks the 
                                       Note: Italics indicate most common pasteurization method for milk.       temperature of a product being cooked in a steam-
                                                                                                                jacketed kettle.
        FS-15-W                Food Entrepreneurship Series  •                                       Purdue extension
                               Food Preservation Methods 
                       
          4             Product characterization       pH (all products have water  FDA regulations                 Example
                                                       activity greater than 0.85)
                        Acidic                         natural pH equal to or less than                             lemons
                                                       4.6
                        Acidified                      formulated pH equal to or less   21 CFR 108.25               tomato salsa with green 
                                                       than 4.6                         21 CFR 114                  peppers & onions
                        Low-Acid                       pH greater than 4.6              21 CFR 108.35               canned green beans
                                                                                        21 CFR 113
                       Note: Some exceptions do exist in these regulations. Please refer to the Code of Federal Regulations for more information about 
                       these exceptions.
                                  of the food, type of container, and size of container.       ally fermented food. Non-fermented products are 
                                  For example, low-acid canned foods (those with a pH          acidified by adding acid—for example, vinegar or 
                                  greater than 4.6and water activity greater than 0.85)        citric acid—to the product. In the past, the majority of 
                                                                                o              acidified foods were fermented in the home, but as 
                                  need to be heated at a high temperature (240 F) to 
                                  ensure destruction of Clostridium botulinum. In most         demand grew for a large, consistent food supply, the 
                                  cases a pressurized cooker is required to reach such         direct addition of acid has become more common. 
                                  high temperatures.                                           This allows food processors to increase quality, con-
                                  Before manufacturing low-acid canned foods, the              sistency, and manufacturing speed. Some common 
                                  food processor must meet U.S. Food and Drug                  non-fermented products widely available today are 
                                  Administration (FDA) regulations. See “Registering           pickled beets and dill pickles (cucumbers that have 
                                  Food Processes and Facilities with the U.S. Food             been pickled.
                                  and Drug Administration” (below) for more informa-           Depending on the microorganisms used in fermen-
                                  tion.                                                        tation (i.e., bacteria and/or yeast), carbohydrates 
                                  Acidifying (Acidified Foods)                                 are converted into acids, gas, or alcohol. Thus, 
                                  Adding acid to a product with a initial pH greater than      fermentation is not solely used on low-acid fruits and 
                                  4.6 in order to reduce it below 4.6 is called acidifica-     vegetables. Fermentation is also commonly used to 
                                  tion. This results in an acidified food. Low-acid fruits     produce dairy products – yogurt and cheese – as 
                                  and vegetables (those with a pH greater than 4.6) are        well as beer and wine, but be aware that these prod-
                                  often preserved by acidification.                            ucts are subject to different regulations than other 
                                                                                               acidified foods.
                                                                                               Before manufacturing acidified foods that are not 
                                                                                               refrigerated, the food processor must meet U.S. 
                                                                                               Food and Drug Administration (FDA) regulations. 
                                                         Cucumbers can be preserved            See “Registering Food Processes and Facilities with 
                                                         by a fermentation or non-             the U.S. Food and Drug Administration” (below) for 
                                                         fermentation process, both of         more information.
                                                         which acidify the cucumbers           Registering Food Processes and 
                                                         and prevent the growth of 
                                                         harmful bacteria.                     Facilities with the U.S. Food and 
                                                                                               Drug Administration
                                                                                               In order to begin processing low-acid canned 
                                                                                               foods or acidified foods, a food processor must 
                                  Acidified fruits and vegetables (sometimes referred          first register the processing location with the U.S. 
                                  to as “pickled foods”) can be either fermented or            Food and Drug Administration (FDA) and submit 
                                  non-fermented. Fermented products are those with             for review a Form 2541 showing the scheduled 
                                  an initial pH greater than 4.6 that are placed in a          process, or food-processing conditions. Prior to this 
                                  brine (saltwater) solution in which bacteria (native or      filing, it is recommended that the scheduled process 
                                  added culture) convert carbohydrates to acid. This           be reviewed by a process authority, which is an 
                                  acidifies the product, making it shelf stable without        individual or group of professionals recognized by 
                                  refrigeration. Sauerkraut is an example of a tradition-      the FDA to have specific expertise in the methods 
                                                                                               used for food preservation. In addition, the processor 
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