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picture1_Performance Ppt 77615 | Chromatography


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File: Performance Ppt 77615 | Chromatography
i introduction i i introduction i introduction introduction ii classification of chromatographic methods ii ii classification of chromatographic methods ii classification of chromatographic methods classification of chromatographic methods iii principle ...

icon picture PPT Filetype Power Point PPT | Posted on 03 Sep 2022 | 3 years ago
Partial capture of text on file.
        Gas Liquid Chromatography
       Here the mobile phase is an unreactive gas ( eg 
       Nitrogen) flowing through a tube.
       And the stationary phase is an involatile liquid 
       held on particles of a solid support.
     In the animation below the red molecules are more soluble 
     in the liquid (or less volatile) than are the green molecules. 
    In practice the Column is contained in a thermostatic oven.  
    (Why ?)
     About 1μL of liquid is  injected into one end of the column.
    As each component reaches the other end it is detected 
    and registered on a chart recorder.
    The Retention Time is characteristic of a particular 
    substance. (for the same column, temperature, gas flow etc.)
     The area under each peak indicates the relative quantities.
        Injection 
        port                     Recorder
                    Oven
                               Detector
                  Column
    Nitrogen 
    cylinder
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...I introduction ii classification of chromatographic methods iii principle chromatography iv high performance liquid hplc v gas gc vi thin layer tlc definition is defined as a procedure by which solutes are separated dynamic differential migration process in system consisting two or more phases one moves continuously given direction and the individual substances exhibit different mobilities reason differences adsorption partition solubility vapor pressure molecular size ionic charge density mobile phase that travels through column transport sample stationary immiscible solid fixed place on support retain analytes within band zone area across analyte distributed zones gradually separate bands progress down chromatograp hy eluant detection method chromatogram to components mixture based distribution coefficients between categorized basis interaction solute either liq sol...

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