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Beer’s Law A student is instructed to determine the concentration is a solution of CoCl2 based on the absorption of light. (spectrophotometer/colorimeter method).The student is provided with a 0.10M CoCl2 solution with which they are to prepare standard solutions with concentrations of 0.020M, 0.040M, 0.060M and 0.080M. Describe the procedure for diluting the 0.10M solution to a concentration of 0.020M using distilled water, a 100ml volumetric flask, and a pipet or buret. Include Specific amounts where appropriate. Solution: 1pt for CoCl ; 1 pt for H O 2 2 MV = MV 1 1 2 2 0.10M(V1) = 0.02M(100ml) V1 = 20ml CoCl2 80ml = H2O Pipet 20ml of the CoCl2 into a volumetric flask. Add enough distilled water to the flask to reach the 100ml marking on the flask. Mix well. Part b. A student takes the 0.10M solution and determines the percent transmittance and the absorbance at various wavelengths. The two graphs below represent the data: b. Identify the optimum wavelength for the analysis. Solution – 1 point: The optimum wavelength is in the range of 490 – 520nm. The actual wavelength is 510nm Part c The student measure the absorbance of the 0.020M, 0.040M, 0.060M, 0.080M and 0.10M solutions. The data are plotted below. c. The absorbance of the unknown solution is 0.275. What is the concentration of the solution? Solution – 1 point: The acceptable range is 0.045M – 0.055M. The actual value is 0.050M. d. Beer’s Law is an expression that includes three factors that determine the amount of light that passes through a solution. Identify two of these factors. Solution: 1 point each factor ID’d and explained A=abc a=molar absorbtivity b=path length of the cuvette c=concentration
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