221x Filetype XLSX File size 0.16 MB Source: trace-recency.org
Sheet 1: How to Use This Tool
Rapid Test for Recent Infection (RTRI) Surveillance Activities and Inventory Tool | ||||||||||||
This comprehensive spreadsheet has been developed to aid RTRI implementors to: 1) Calculate the laboratory consumables needed for trainings and specimen panel production: Quality Control (QC) panels, Training Panels (TP), and Proficiency Testing (PT) panels. 2) Maintain a laboratory consumables inventory log to ensure sufficient supply for upcoming additional TOTs and step-down trainings 3) Monitor national stock supply of RTRI tests and determine when to place next order 4) Maintain QC, PT, TP panel inventory to ensure there are no stock-outs needed for testing quality monitoring, trainings, and a proficiency testing program 5) Maintain an inventory and testing results log of blood bank samples used to make specimen panels for the RTRI program 6) Determine the estimated costs associated with implementing a recency surveillance program Instructions are provided on each tab on how to fill in the necessary information, how frequently the activities should be performed, and information for consideration. The intended users for this tool include, but not limited to, National Reference Lab (NRL) staff, MoH staff, RTRI implementing partners, and study managers responsible for monitoring and maintaining supplies and tests needed for RTRI testing. |
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CALCULATING LABORATORY CONSUMABLES FOR RAPID TEST FOR HIV-1 RECENT INFECTION (RTRI) TRAININGS |
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INSTRUCTIONS: This calculator is used to estimate the total number of RTRI kits and other lab-related consumables needed for recency training of trainers (ToTs), DTS QC, and plasma training panel preparation and trainings. The costs will vary depending on the country and pricing agreement with vendors. Please enter the relevant information in cells C12:C19 (higlighted in yellow) and the supplies and costs will be calculated automatically below. Note: If the country is considering only plasma samples for their QC, TP and PT panel productions, then please contact CDC ILB ISMEs for the plasma version only of this calculator. Additionally, please contact ILB to determine if a prodcut can be substituted. |
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TRAINING INPUT VARIABLES | ||||||||
Number of TOTs participants | ||||||||
Total number of testers to be trained at step-down trainings | ||||||||
Number of testers at each step-down training (if split) | ||||||||
Name of test kit (select from drop-down list) | Maxim Swift HIV-1 Rapid Recency test (20 test packing) | |||||||
Number of plasma units collected | ||||||||
Number of QC panel set to prepare | ||||||||
Number of training panel set to prepare | ||||||||
*ALL pricing in this calculator is an estimate and should be used for preliminary budgeting purposes only. For actual pricing, please contact each manufacturer for a specific quote. | ||||||||
CONSUMABLES FOR PURCHASING | ||||||||
Item Description | Application | Unit | Units Needed |
Items Received | Estimated Price per unit* |
Total Estimated Price* | AVAILABLE IN COUNTRY? | |
Maxim Swift HIV-1 Rapid Recency test (20 test packing) | Reagent kit | Test | $3.95 | #VALUE! | ||||
Desk Model Biohazard Bag Holder for tabletop waste (example, Bel-Art Scienceware Poxygrid, Fisher #01-815-2) | Waste Disposal | each | $32.00 | |||||
Polypropylene Biohazard Autoclave Bags; Size: 8 x 12in for tabletop waste (example, Fisher #03-410-591) | Waste Disposal | pack of 100 | $28.55 | |||||
Polypropylene Biohazard Autoclave Bags, large red or clear with biohazard symbol (example, Fisher #S42905) | Waste Disposal | pack of 200 | $361.00 | |||||
Powder-Free Nitrile Exam Gloves with Aloe, Small to X-Large size (example, Fisher #19-050-550A--D) | PPE | pack of 100 pairs | $49.50 | |||||
Lab coat (Small to X-Large size), typically disposable (example, Fisher #19-043057--60) | PPE | case of 25 | $242.50 | |||||
Disposable face mask (example, VWR Catalog Number: 76451-956) | PPE | case of 500 | $423.88 | For mitigation of COVID-19, 1-2 /person/day. The calculation is based on a 3 day training. Note: Mask worn during practical session should be treated as biohazadous waste. | ||||
Sarstedt Microtubes 2.0ml with enclosed cap, 250/bag, Sartstedt #72.694.007 | Panel Prep | 250/bag | $90.25 | |||||
Microtube racks to accommodate 96 2.0ml tubes (example, Fisher #05-541-38) | Panel Prep | pack of 5 | $101.00 | |||||
Specimen tube labels, Laser Cryo-Babies 0.94 x 0.50" 2,380/pk, Diversified Biotech #LCRY-2380 | Panel Prep | 2380/pack | $69.00 | |||||
Specimen box labels, Laser Cryo-Tags 2.625 x 1.0" 600/pk, Diversified Biotech #LCRY-1258 | Panel Prep | 600/pack | $69.00 | |||||
Repeat Volume Pipettor (example, Thermo Scientific™ Finnpipette Stepper Pipette, Fisher #21-377-147) | Panel Prep | each | $536.50 | |||||
Repeat Volume Pipettor Tips, 5ml (example, Thermo Scientific Finntip, pack of 50, Fisher #21-377-154) | Panel Prep | 50/pack | $86.50 | |||||
Repeat Volume Pipettor Tips, 0.5ml (example Thermo Scientific Finntip, pack of 100, Fisher #21-377-148) | DTS panel prep | 100/pack | $164.80 | |||||
Repeat Volume Pipettor Tips, 50ml (example Thermo Scientific Finntip, pack of 10, Fisher #21-377-160) | Panel Prep | 10/pack | $24.70 | |||||
50 mL Centrifuge tubes, polypropylene (example, Fisher #S99412) | Panel Prep | 50/pack | $15.00 | |||||
250 mL plasma storage bottles (example, Fisher #02-893B) | Panel Prep | 12/pack | $200.50 | |||||
PBS with 0.05% Tween 20, pH 7.4 powder (Sigma-Aldrich #P3563) | DTS panel prep | 10/box | $57.20 | |||||
Green food coloring dye, local market purchase | DTS panel prep | each | $2.00 | |||||
Disposable transfer pipettes (Samco #282 transfer pipet, Fisher #13-711-43) | DTS panel prep | 500/box | $71.00 | |||||
10 mL Serological pipette (example, Fisher #12-567-615) | Panel Prep | 200/case | $128.00 | |||||
Pipet Filler (example, Fisherbrand™ Pipet Controller #FB14955202) | Panel Prep | each | $367.00 | |||||
Cryoboxes, 9x9in 81-place (example, Fisher #12-565-182) | Panel Storage | case of 6 | $54.60 | |||||
Cryoboxes, 5x5in 25-place (example, Fisher #12-565-183) | Training Panel | case of 24 | $187.90 | |||||
absorbant surface protectors or bench pad/linen saver (example, Fisher #14-206-62) | Biosafety | 50 per pack | $83.50 | |||||
70% Alcohol or Bleach-Based Disinfectant (example, Fisher #04-355-150) | Biosafety | bottle | $35.27 | |||||
Chemically Resistant Permanent Markers, ultra fine tip (example, Fisher #22-026-700) | Labeling, training | pack of 12 | $97.00 | |||||
Pens, black ballpoint (example, Staples #2072214) | Record keeping | pack of 12 | $2.09 | |||||
Lab Timer, multi-channel (example, Fisher #S41782) | RTRI testing | each | $37.00 | |||||
Paper towels | RTRI testing | roll each | $2.00 | |||||
Freezer, -20C, upright manual defrost, ~566L capacity, 230 V/50 Hz (example, Fisher #20LFEETSV) | NRL QC/TP Storage | each | $3,006.00 | |||||
Surgical Masks (example, Fisher #19-088-879) | PPE | pack of 500 | $95.00 | |||||
Hand Sanitizer (example, Fisher #23-001-729) | PPE | each | $15.80 | |||||
Disposable Face Shields (example, Fisher #19-181-600B) | PPE | pack of 25 | $66.00 | |||||
Optional (verify lab has deionized water source and vacuum filtration): | ||||||||
1L Disposable 0.2 uM filter flask (Fisher #09-761-104) | PT buffer | case of 12 | $351.20 | |||||
Reagent grade deionized water, (Fisher #23-751610) | DTS | 1 gal | $50.60 | |||||
TOTAL: | #VALUE! | |||||||
1 |
INVENTORY LOG OF LABORATORY CONSUMABLES FOR RTRI TRAININGS | ||||||||||||||||||||
INSTRUCTIONS: The spreadsheet should be used to maintain an ongoing inventory of laboratory supplies needed for additional ToT, step-down training and panel preparation (QC, TP and PT panels). This inventory tool should be completed EVERY MONTH and AFTER EVERY TRAINING. It should be used together with the Consumables Calculator (previous tab) to help determine preparedness status when planning for training and panel preparation activities. Take note of expiry dates of the supplies and exercise the first in/first out (FIFO) and/or first expired/first out (FEFO) rules. Add INVENTORY DATE into the yellow box and add columns as needed. |
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LABORATORY CONSUMABLES | INVENTORY LOG | |||||||||||||||||||
Inventory Date: | Inventory Date: | Inventory Date: | Inventory Date: | Inventory Date: | Inventory Date: | |||||||||||||||
Item Desciption | Application | Unit | Quantity Available |
Expiry Date (If Applicable) |
Comments | Quantity Available |
Expiry Date (If Applicable) |
Comments | Quantity Available |
Expiry Date (If Applicable) |
Comments | Quantity Available |
Expiry Date (If Applicable) |
Comments | Quantity Available |
Expiry Date (If Applicable) |
Comments | Quantity Available |
Expiry Date (If Applicable) |
Comments |
Asante HIV-1 Rapid Recency test (100 test packaging) | Reagent Kit | Test | ||||||||||||||||||
Asante HIV-1 Rapid Recency test (20 test packaging) | Reagent Kit | Test | ||||||||||||||||||
Maxim Swift HIV-1 Rapid Recency test (20 test packing) | Reagent Kit | Test | ||||||||||||||||||
Desk Model Biohazard Bag Holder for tabletop waste | Waste disposal | Each | ||||||||||||||||||
Polypropylene Biohazard Autoclave Bags; Size: 8 x 12in for tabletop waste | Waste disposal | pack of 100 | ||||||||||||||||||
Polypropylene Biohazard Autoclave Bags, large red or clear with biohazard symbol | Waste disposal | pack of 200 | ||||||||||||||||||
Powder-Free Nitrile Exam Gloves with Aloe, SMALL | PPE | Pack of 100 pairs | ||||||||||||||||||
Powder-Free Nitrile Exam Gloves with Aloe, MEDIUM | PPE | Pack of 100 pairs | ||||||||||||||||||
Powder-Free Nitrile Exam Gloves with Aloe, LARGE | PPE | Pack of 100 pairs | ||||||||||||||||||
Powder-Free Nitrile Exam Gloves with Aloe, X-LARGE | PPE | Pack of 100 pairs | ||||||||||||||||||
Disposable Lab Coat, SMALL | PPE | case of 25 | ||||||||||||||||||
Disposable Lab Coat, MEDIUM | PPE | case of 25 | ||||||||||||||||||
Disposable Lab Coat, LARGE | PPE | case of 25 | ||||||||||||||||||
Disposable Lab Coat, X-LARGE | PPE | case of 25 | ||||||||||||||||||
Disposable face mask (example, VWR Catalog Number: 76451-956) | PPE | case of 500 | ||||||||||||||||||
Sarstedt Microtubes 2.0ml with enclosed cap, 250/bag | Panel prep | 250/bag | ||||||||||||||||||
Microtube racks to accommodate 96 2.0ml tubes | Panel prep | pack of 5 or single | ||||||||||||||||||
Specimen tube labels, Laser Cryo-Babies 0.94 x 0.50" 2,380/pk, | Panel prep | 2380/pack | ||||||||||||||||||
Specimen box labels, Laser Cryo-Tags 2.625 x 1.0" 600/pk, | Panel prep | 600/pck | ||||||||||||||||||
Repeat Volume Pipettor Tips, 5ml (example, Thermo Scientific Finntip, pack of 50, Fisher #21-377-154) | Panel Prep | 50/pack | ||||||||||||||||||
Repeat Volume Pipettor Tips, 5ml (example, Thermo Scientific Finntip, pack of 50, Fisher #21-377-154) | Panel Prep | 50/pack | ||||||||||||||||||
Repeat Volume Pipettor Tips, 0.5ml (example Thermo Scientific Finntip, pack of 100, Fisher #21-377-148) | DTS panel prep | 100/pack | ||||||||||||||||||
Repeat Volume Pipettor Tips, 50ml (example Thermo Scientific Finntip, pack of 10, Fisher #21-377-160) | Panel Prep | 10/pack | ||||||||||||||||||
50 mL Centrifuge tubes, polypropylene | Panel prep | 50/pack | ||||||||||||||||||
250 mL plasma storage bottles (example, Fisher #02-893B) | Panel Prep | 12/pack | ||||||||||||||||||
PBS with 0.05% Tween 20, pH 7.4 powder (Sigma-Aldrich #P3563) | Panel prep | 10/box | ||||||||||||||||||
Green food coloring dye, local market purchase | Panel prep | each | ||||||||||||||||||
Disposable transfer pipettes (Samco #282 transfer pipet | Panel prep | 500/box | ||||||||||||||||||
Disposable transfer pipettes (Samco #282 transfer pipet, Fisher #13-711-43) | DTS panel prep | 500/box | ||||||||||||||||||
10 mL Serological pipette | Panel prep | 200/case | ||||||||||||||||||
Cryoboxes, 9x9in 81-place | Multi use | each | ||||||||||||||||||
Cryoboxes, 5x5in 25-place (example, Fisher #12-565-183) | Training Panel | case of 24 | ||||||||||||||||||
Absorbant surface protectors or bench pad/linen saver | Biosafety | 50/pack | ||||||||||||||||||
70% Alcohol or Bleach-Based Disinfectant | Biosafety | bottle | ||||||||||||||||||
Chemically Resistant Permanent Markers, ultra fine tip | Labelling-training | each | ||||||||||||||||||
Pens, black ballpoint | Record Keeping | each | ||||||||||||||||||
Lab Timer, multi-channel/Single channel | RTRI training | each | ||||||||||||||||||
Paper towels | RTRI training | Roll | ||||||||||||||||||
Printing paper | RTRI training | Pack | ||||||||||||||||||
RTRI Job Aids | RTRI training | Each | ||||||||||||||||||
Surgical Masks (example, Fisher #19-088-879) | PPE | pack of 500 | ||||||||||||||||||
Hand Sanitizer (example, Fisher #23-001-729) | PPE | each | ||||||||||||||||||
Disposable Face Shields (example, Fisher #19-181-600B) | PPE | pack of 25 | ||||||||||||||||||
Optional: 1L Disposable 0.2 uM filter flask (Fisher #09-761-104) | PT buffer | case of 12 | ||||||||||||||||||
Optional: Reagent grade deionized water, (Fisher #23-751610) | DTS | 1 gal |
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