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                            Nebraska Beef Cattle Reports                                                                                                Animal Science Department 
                            January 2008 
                            EEffffects of Corn ects of Corn PrProcessing and ocessing and WWet Distillers et Distillers GrGrains on Nutrient ains on Nutrient 
                            Metabolism Metabolism 
                            Mark E. Corrigan 
                            University of Nebraska-Lincoln 
                            Galen E. Erickson 
                            University of Nebraska-Lincoln, gerickson4@unl.edu 
                            Terry J. Klopfenstein 
                            University of Nebraska-Lincoln, tklopfenstein1@unl.edu 
                            Nathan F. Meyer 
                            University of Nebraska-Lincoln, nmeyer2@unl.edu 
                            Follow this and additional works at: https://digitalcommons.unl.edu/animalscinbcr 
                                  Part of the Animal Sciences Commons 
                            Corrigan, Mark E.; Erickson, Galen E.; Klopfenstein, Terry J.; and Meyer, Nathan F., "Effects of Corn 
                            Processing and Wet Distillers Grains on Nutrient Metabolism" (2008). Nebraska Beef Cattle Reports. 17. 
                            https://digitalcommons.unl.edu/animalscinbcr/17 
                            This Article is brought to you for free and open access by the Animal Science Department at 
                            DigitalCommons@University of Nebraska - Lincoln. It has been accepted for inclusion in Nebraska Beef Cattle 
                            Reports by an authorized administrator of DigitalCommons@University of Nebraska - Lincoln. 
                        Effects of Corn Processing and Wet Distillers Grains 
                                                     on Nutrient Metabolism
                         Mark E. Corrigan                  attempt to elucidate the reasons for         Table 1.  Diets fed to steers in the digestibility 
                         Galen E. Erickson                 the interaction observed in the finish-             experiment evaluating wet distillers 
                                                                                                               grains plus solubles (WDGS) when fed 
                       Terry J. Klopfenstein               ing trial.                                                                          a
                                                                                                               with different corn processing methods  
                                           1                                                                   (% of diet DM).
                         Nathan F. Meyer
                                                                          Procedure                     Ingredient        0% WDGS  40% WDGS
                             Summary                          Seven ruminally cannulated steers         DRC, HMC, or SFC     82.5       47.5
                                                                                                        WDGS                  0.0       40.0
                                                           were used in a six-period cross-over         Alfalfa hay           7.5        7.5
                 Seven ruminally cannulated steers         study. A  × 2 factorial treatment           Molasses              5.0        0.0
              were used in a metabolism experiment         structure was used. The first factor         Supplement            5.0        5.0
              to determine the effects of distillers       was corn processing method (DRC,             aDRC = dry-rolled corn, HMC = high-moisture 
              grains level (0% or 40% of diet DM) on       HMC, or SFC), and the second fac-            corn, and SFC = steam-flaked corn.
              diet digestibility and rumen metabolism      tor was WDGS inclusion level (0%             cannula. Data were collected using 
              in diets consisting of dry-rolled, high-     or 40% of diet DM; Table 1). Steers          a computer and software (Labtech, 
              moisture, or steam-flaked corn. Intakes      were assigned randomly to one of             Wilmington, Mass.) that collected 
              were greater while digestibility was         two groups. Group 1 steers were as-          readings every 6 seconds and averaged 
              reduced for DM and organic matter in         signed to diets containing 0% WDGS           those for each minute for the 5 days 
              steers fed 40% compared to 0% distillers     during the first three periods and           collection within each period.
              grains. Average and maximum pH were          diets containing 40% WDGS dur-                  Data were analyzed as a six- period 
              less for steers fed 40% distillers grains,   ing the final three periods, while           crossover design using the MIXED 
              but pH change and variance were also         group 2 steers were assigned to diets        procedure of SAS (SAS Inst. Inc.). 
              less for 40% compared to 0% WDGS.            containing 40% WDGS during the               Period was included in the model as 
              Feeding WDGS does not increase rumen         first three periods and diets contain-       a fixed effect and the random effect 
              pH, but does decrease variance. Starch       ing 0% WDGS during the final three           was steer. No corn processing method 
              intakes were equal whether 0 or 40%          periods A two-week transition period         × WDGS inclusion level interactions 
              WDGS were fed because cattle fed 40%         was included between periods three           were observed (P > 0.20) for any vari-
              WDGS had greater DMI, which may              and four during which group 2 steers         ables, so only the main effects of corn 
              influence rumen pH.                          were fed decreasing levels of WGDS as        processing method and WDGS inclu-
                                                           follows: day 1 to 4: 0%, day 5 to 10:       sion level are presented. As a result, 
                           Introduction                    20%, and day 11 to 14: 10% WDGS,             main effects were analyzed and sta-
                                                           DM. During that transition, group 1          tistics presented with the interaction 
                 In a previously reported study            steers were switched immediately to          term removed from the model.
              (2007 Nebraska Beef Report, pp. -          diets containing 40% WDGS and all 
              5), diets based on dry-rolled corn          diets were based on an equal mixture                         Results
              (DRC), high-moisture corn (HMC),             of DRC, HMC, and SFC.
              or steam-flaked corn (SFC) were fed.            Period duration was 20 days                  Data for nutrient intake and 
              Corn was replaced with increasing            and consisted of a 15-day adapta-            digestibility are presented in Table 
              amounts of wet distillers grains with        tion period followed by a 5 day fecal        2. No corn processing method × 
              soluble (WDGS; 0%, 15%, 27.5%,               sample and pH data collection period.        WDGS inclusion level interactions for 
              or 40% of diet DM). No effect of             Chromic oxide (7.5g/dose) was dosed          nutrient intake or digestibility were 
              WDGS level on feed efficiency was            intraruminally at 0800 and 2000 daily        observed (P > 0.4), so main effects 
              observed in SFC based diets, and             beginning on day 11 in each period.          will be discussed. Corn process-
              ADG was reduced when 27.5% or 40%            Fecal samples were collected daily at        ing method did not affect intake or 
              WDGS were fed. In DRC and HMC                0800, 1400, and 2000, composited by          digestibility of DM or organic matter 
              based diets however, feed efficiency         period, and analyzed for chromium            (OM). Digestibility of neutral deter-
              improved linearly with increasing            content to determine nutrient digest-        gent fiber (NDF) was similar between 
              WDGS level, and ADG increased.               ibility. Steers were fed once daily at       corn processing methods; however, 
              The current study was conducted to           070 and, if present, feed refusals were     cattle fed HMC consumed slightly less 
              determine the effects of corn pro-           also collected at this time. Continuous      NDF (P < 0.05). Starch intake was not 
              cessing method and WDGS level                ruminal pH measurements were taken           impacted by corn processing method, 
              on nutrient digestion and ruminal            using pH probes that were suspended          but starch digestibility was greater 
              fermentation characteristics in an           in the rumen fluid via the rumen                               (Continued on next page)
               © The Board of Regents of the University of Nebraska.  All rights reserved.                2008 Nebraska Beef Report  — Page 4 
                (P < 0.06) for SFC compared to both               Table 2.  Effect of corn processing method and wet distillers grains plus solubles (WDGS) level on 
                DRC and HMC. Feeding WDGS                                  nutrient intake and digestibility.
                                                                                                               a                                       b
                increased intake of DM and OM but                                       Corn processing method       WDGS level                 P-value
                decreased the digestibility percent-              Item                    DRC    HMC      SFC        0%     40%        Process   WDGS  Inter
                age of both. While NDF digestibility              DM
                was not impacted by feeding WDGS,                    Intake, lb/day       20.8    19.5    20.7      18.4    22.         0.25      0.01     0.9
                cattle fed 40% WDGS consumed                         Digestibility, %     78.7    78.8    81.4      81.8    77.5         0.1      0.08     0.96
                more NDF (P < 0.01) because WDGS                  OM
                contain more NDF than corn. Starch                   Intake, lb/day       20.1    19.0    19.9      18.0    21.4         0.4      0.02     0.94
                                                                     Digestibility, %     80.7    80.9    8.      84.0    79.         0.2      0.05     0.94
                is removed during the production of               NDF
                                                                                               c       d       c
                WDGS. Despite lower dietary starch                   Intake, lb/day        .94    .5    .74      2.9    4.97        0.02      0.01     0.64
                percentage in the 40% WDGS diets,                    Digestibility, %     49.4    47.2    50.9      47.8    50.5         0.80      0.72     0.7
                                                                  Starch
                feeding WDGS did not reduce total                    Intake, lb/day       12.0    11.2    11.7      11.6    11.7         0.65      0.90     0.77
                                                                                              c       c       d
                starch intake (P = 0.90) and did not                 Digestibility, %     95.5    96.5    99.1      96.6    97.5         0.04      0.57     0.4
                impact starch digestibility.                      aDRC = dry-rolled corn, HMC = high-moisture corn, and SFC = steam-flaked corn.
                                                                  b
                   Rumen pH data are presented in                  P-value where Process = corn processing method; WDGS = wet distillers grains plus solubles level; 
                                                                  Inter = interaction between corn processing method and WDGS level.
                Table . No corn processing method ×              c,dMeans with different superscripts differ (P < 0.06).
                WDGS inclusion level interactions for 
                rumen pH data were observed                       Table 3.  Effect of corn processing method and wet distillers grains plus solubles (WDGS) level on 
                (P > 0.27), so only the main effects are                   rumen pH.
                discussed. An effect of corn process-                                                          a                                       b
                ing method on maximum pH                                                Corn processing method       WDGS level                 P-value
                (P = 0.04), the magnitude of pH                   Item                    DRC    HMC      SFC        0%     40%        Process   WDGS  Inter
                change (P = 0.05), and the variance               Average pH               5.5    5.56    5.44      5.61    5.41        0.27      0.12     0.51
                                                                                               c       d       d
                of the ruminal pH (P < 0.02) was                  Maximum pH               6.22    6.41    6.50      6.50    6.26        0.04      0.07     0.49
                observed. Steers fed DRC had lower                Minimum pH               5.00    5.06    4.9      5.01    4.98        0.6      0.80     0.82
                                                                                               c       c       d
                maximum rumen pH values com-                      pH change                1.21    1.4    1.56      1.50    1.25        0.05      0.08     0.27
                                                                                                c       c       d
                pared to steers fed HMC and SFC                   pH variance              0.070   0.109   0.161     0.140   0.087       0.02      0.09     0.56
                (P < 0.10). Interestingly, average and            Area under curve (magnitude of pH < 5.6 or 5. by minute)
                                                                                            c       cd      d
                                                                     < 5.6               260     07     98       245     99           0.10      0.07     0.62
                minimum rumen pH values were                                                c       cd      d
                                                                     < 5.                67     11     149        76     144           0.06      0.08     0.97
                not different between the three corn              aDRC = dry-rolled corn, HMC = high-moisture corn, and SFC = steam-flaked corn.
                processing methods. This led to both              b
                                                                   P-value where Process = corn processing method; WDGS = wet distillers grains plus solubles level; 
                magnitude of pH change and variance               Inter = interaction between corn processing method and WDGS level.
                of pH being numerically lowest in                 c,dMeans with different superscripts differ (P < 0.10).
                steers fed DRC. Although not signifi-
                cant, steers fed HMC had a numeri-                with HMC fed steers being intermedi-              was 18.9 lb/day for 0% WDGS and 
                cally greater pH change and variance              ate. Interestingly, feeding 40% WDGS              22.4 lb/day for 40% WDGS in SFC 
                than steers fed DRC. Steers fed SFC               resulted in greater time spent below a            diets. Despite no interaction between 
                experienced a pH change and variance              rumen pH of 5.6 or 5. compared to                corn processing method and WDGS 
                that was greater (P < 0.10) than steers           cattle fed 0% WDGS. These data agree              inclusion, starch digestibility was 
                fed DRC or HMC. Inclusion level of                with previous research evaluating                 consistent between WDGS levels in 
                WDGS also affected rumen pH. Inter-               rumen metabolism with and without                 HMC and SFC diets, with less than 
                estingly, steers fed 0% WDGS tended               WDGS (2007 Nebraska Beef Report,                  0. percentage unit change between 
                to have greater average pH (P < 0.12),            pp. 9-42).                                       0 and 40% WDGS within each corn 
                maximum pH (P = 0.07), pH change                      The lack of an interaction between            processing method. However, steers 
                (P = 0.08), and pH variance (P = 0.09)            corn processing method and WDGS                   fed 0% WDGS with DRC had a 
                compared to steers fed 40% WDGS.                  inclusion level in this trial does not            starch digestibility of 9.9% which 
                As was the case with corn processing              explain the interaction observed in               was numerically lower than the 40% 
                method, minimum pH was not dif-                   previous finishing trials. However,               WDGS with DRC treatment (97.0%).
                ferent between 0% and 40% WDGS.                   DMI was markedly reduced when 
                Area under the curve for pH of 5.6                WDGS were fed at 40% of the diet 
                                                                                                                        1
                and 5. followed similar trends as                in SFC-based diets in the previous                     Mark E. Corrigan, graduate student; Galen 
                maximum pH. Steers fed SFC had a                  finishing study. A similar intake                 E. Erickson, associate professor; Terry J. Klopfen-
                rumen pH below 5.6 and 5. for more               response was not observed in this                 stein, professor; Nathan F. Meyer, research tech-
                                                                                                                    nician; Department of Animal Science, Lincoln.
                minutes compared to steers fed DRC,               metabolism experiment as DMI 
                Page 44 — 2008 Nebraska Beef Report                                          © The Board of Regents of the University of Nebraska.  All rights reserved.
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...View metadata citation and similar papers at core ac uk brought to you by provided digitalcommons university of nebraska univuniversity nebrersity lincoln aska univdigitalcommons beef cattle reports animal science department january eeffffects corn ects prprocessing ocessing wwet distillers et grgrains on nutrient ains metabolism mark e corrigan galen erickson gerickson unl edu terry j klopfenstein tklopfenstein nathan f meyer nmeyer follow this additional works https animalscinbcr part the sciences commons effects processing wet grains article is for free open access it has been accepted inclusion in an authorized administrator attempt elucidate reasons table diets fed steers digestibility interaction observed finish experiment evaluating plus solubles wdgs when ing trial a with different methods diet dm procedure ingredient summary seven ruminally cannulated drc hmc or sfc were used six period cross over alfalfa hay study factorial treatment molasses structure was first factor supple...

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